Global mean sea level: indicator of climate change?

1. L. A. Sherman and R. M. Brown, in Comprehensive Virology, H. Frankel-Conrat and R. R. Wagner, Eds. (Plenum, New York, 1978), vol. 12, p. 145. 2. P. A. Lemke, Annu. Rev. Microbiol. 30, 105 (1976); J. A. Dodds, Experientia 35,.440 (1979). 3. A. Skotnicki, A. J. Gibbs, N. G. Wrigley, Virology 75, 457 (1976); J. A. Dodds and A. Cole, ibid. 100, 156 (1980); A. Cole, J. A. Dodds, R. I. Hamilton, ibid., p. 166; L. H. Stanker, L. R. Hoffmnan, R. MacLeod, ibid. 114, 357 (1981). 4. R. H. Meints, J. L. Van Etten, D. Kuczmarski, K. Lee, B. Ang, ibid. 113, 698 (1981); J. L. Van Etten, R. H. Meints, D. E. Burbank, D. Kuczmarski, D. A. Cuppels, L. C. Lane, ibid., p. 704. 5. J. L. Van Etten et al., Proc. Natl. Acad. Sci. U.S.A.. 79, 3867 (1982). 6. S. J. Karakashian and M. W. Karakashian, Evolution 19, 368 (1965); D. S. Weis, J. Protozool. 25, 366 (1978). 7. E. Jolley and D. C. Smith, New Phytol. 81, 637 (1978). 8. The algae were grown on Bold's basal medium [IH. W. Nicols and M. C. Bold, J. Phycol. 1, 34 (1965)] modified by the addition of 0.5 percent sucrose and 0.1 percent proteose peptone (MBBM). Fifty-milliliter cultures were inoculated with algae (I x 106 per milliliter) and grown at 25°C on a rotary shaker (150 rev/min) in continuous light (30 p.E/m2 per second). 9. The Chlorella cultures were grown to a density of 5 x 106 cells per milliliter and inoculated with virus sterilized with a 0.4-p.m Nuclepore filter at a multiplicity of infection of 5 to 10. 10. A. Gibbs, A. H. Skotnicki, J. E. Gardiner, E. S. Walker, M. Hollings, Virology 64, 571 (1975); L. Oliveira and T. Bisalputra, Ann. Bot. 42, 439 (1978); J. A. Mayer and F. J. R. Taylor, Nature (London) 281, 299 (1979); B. V. Gromov and K. A. Mamkaeva, Arch. Hydrobiol. Suppl. 60, 252 (1981). 11. We thank D. Chapman, T. Hohman, D. Weis, and L. Muscatine for providing Chlorella cultures and M. Blrakke and L. Lane for helpful comments. This research was supported in part by the Research Council of the University of Nebraska, Lincoln, and by NIH biomedical research support grant RR-07055. This report is published with the approval of the director as paper 6962, journal series, Nebraska Agricultural Experiment Station.